DCs诱导特异性CTL抗非霍奇金淋巴瘤作用的研究

Study of the effect of DCs and tumor-specific CTLs induced by DCs on non-Hodgkin's lymphoma

目的:探讨树突状细胞(dendritic cells,DCs)及经DCs诱导的肿瘤特异性CTL(cytotoxic T lympho-cyte,CTL)对杀伤非霍奇金淋巴瘤(NHL)细胞的作用。方法:分选DCs,NHL细胞株(Raji)冲击DCs,诱导产生CTL,加入荧光标记的抗体100μl(分别为抗CD11a、CD86、CD83的单抗),FACScan检测其表型,MTT检测其刺激淋巴细胞的能力。LDH释放实验检测DC诱导的CTL的杀伤作用。结果:DC可刺激T细胞增殖;CTL对Raji细胞有杀伤作用,CTL与Raji细胞比例为100:1、10:1时,对Raji细胞的杀伤率为(57.1±3.7)%、(35.8±6.2)%,两者有显著差异(P<0.05)。结论:DC及CTL对NHL能产生高效的杀伤作用。

Objective： To investigate the effect of DCs and tumor-specific CTLs induced by DCs on the anti-lymphoma activity of cytotoxic T lymphocytes.Methods： DCs was seperated from healthy human peripheral blood mononuclear cells.DC was attacked by Raji to induce lymphoma-specific CTL.DC phenotype was determined by the flow cytometry.The ability of DC to stimulate lymphocytes and to phagocytize were determined by MTT assay test.DC and T lymphocytes were cultured together to induce lymphoma-specific CTL.We used LDH release assay to measure CTL cytotoxicity.Results：DC coued stimulate the activity of T cell proliferation in a dose dependent manner,and was related with the DC costimulatory molecule expression.CTL induced by DC had the killing effect against lymphoma cells.As the ratio of CTL and lynphoma cell was 100：1 and 10：1,the lyphoma cell-killing rates were（57.1±3.7）% and（35.8±6.2）%,both statistically significant（P0.05）.Conclusion：DC and CTL play an important role in immunotherapy in lyphoma,and have a potential applications in clinic.