E2F3在喉鳞状细胞癌中的表达及临床意义

The expression and clinical significance of E2F3 in laryngeal squamous cell carcinoma

目的:探讨喉鳞癌中E2F3的表达与临床意义,为喉鳞癌的诊断、评估及治疗寻找辅助生物学指标。方法:采用免疫组化方法检测喉鳞癌组织及癌旁正常切缘组织中E2F3表达,结合喉鳞癌各临床参数,用SPSS16.0软件包进行统计分析E2F3表达情况与喉鳞癌各临床特征之间的关系。结果:E2F3在喉鳞癌与癌旁正常切缘中的阳性表达率分别为90.44%(123/136)和27.85%(22/79),差异具有显著统计学意义(P<0.001);E2F3高表达与喉鳞癌患者年龄、临床分期及分型均相关(P<0.05)。结论:E2F3核表达可能参与喉鳞癌发生发展过程;E2F3浆表达可能贯穿于喉鳞癌发生的整体过程。

Objective：To study the expression and clinical significance of E2F3 in laryngeal squamous cell carcinoma.Methods：Immunohistochemical staining was used to detect the expression of E2F3 protein in 136 laryngeal squamous cell carcinoma tissues and 79 adjacent normal tissues.The correlations of E2F3 with clinicopathological factors were analyzed.Results：The positive rates of E2F3 in the adjacent normal tissues and laryngeal squamous cell carcinoma tissues were 90.44%（123/136） and 27.85%（22/79） respectively.The expression of E2F3 in laryngeal squamous cell carcinoma was higher than that in normal mucosa（P0.01）.The expression of E2F3 in laryngeal squamous cell carcinoma tissues was correlated with the clinical stage,age,type,and differentiation degree（P0.05）.And it was not correlated with the T stage,lymph node metastasis,smoking and gender（P0.05）.In addition,very high level expression of nuclear E2F3 was found in almost all subglottic laryngeal squamous cell carcinomas.Conclusion：The expression of E2F3 was highly up-regulated in laryngeal squamous cell carcinoma tissue.It had a relationship with the clinical stage,age,type,and differentiation degree.It may have an important significance on predicting the invasion of laryngeal squamous cell carcinomas,and may permit the laryngeal tumorigenesis and progression.