摘要
以金桂茎段为试验材料,采用正交试验设计方案,探讨大量元素和不同生长调节物质对金桂诱导、增殖以及生根过程中的影响。结果表明:金桂的诱导培养基以MS+6-BA2.5mg/L+NAA0.5mg/L较为适宜;增殖培养基以B5+6-BA3.0mg/L+NAA0.4mg/L为最佳;生根培养基是:改良MS+IBA0.5mg/L+NAA0.02mg/L,生根率可达85%以上。
In order to get the optimal medium to culture Osmanthus fragrans.,semi-ligneous stem sections of a dominant tree in a mature forest were used as explants and orthogonal with the compound of three elements（macroelements,BA and NAA） with three levels was adopted in this study.The results showed that the optimum medium for regeneration bud culture were MS ＋ 6-BA2.5mg/L ＋NAA0.5mg/L,the medium for sub-propagation were B5 ＋ 6-BA3.0mg/L ＋NAA0.4mg/L,improved MS ＋ IBA0.5mg/L＋ NAA0.02mg/L was successful rooting.It was that ratio of the growth rooting reached 85%.
出处
《安徽农学通报》
2012年第8期27-27,165,共2页
Anhui Agricultural Science Bulletin
关键词
桂花
茎段
快速繁殖
Osmanthus fragrans
Stem section
Rapid propagation
