电针治疗对膝骨关节炎兔膝关节软骨细胞凋亡的影响

Study on the effect of electroacupuncture on knee joint chondrocyte apoptosis in rabbits with knee osteoarthritis

目的:观察电针治疗对膝骨关节炎兔膝关节软骨细胞凋亡的影响,探讨电针治疗膝骨关节炎的作用机制。方法:将30只健康新西兰大白兔随机分为正常对照组、模型对照组和电针治疗组,每组10只。采用Hulth法对模型对照组及电针治疗组实验兔左膝关节进行造模,造模后第5周开始对电针治疗组实验兔进行电针治疗,正常对照组、模型对照组同期正常饲养,不作任何治疗。治疗4周后同时处死3组实验兔,取左膝关节股骨端制成切片,观察各组实验兔软骨组织形态及软骨细胞凋亡情况。结果:①软骨组织形态。正常对照组软骨表层光滑、平整,4层结构清晰,软骨细胞分布均匀、无簇聚,潮线完整;模型对照组关节软骨厚度减小,表面毛糙,可见明显纤维化、裂隙、软骨细胞簇聚,潮线断裂;电针治疗组关节软骨厚度减小,表面不光滑,少见纤维化、裂隙、软骨细胞簇聚,潮线断裂。②软骨细胞凋亡情况。3组实验兔软骨细胞凋亡率比较,差异有统计学意义(F=126.753,P=0.000);进一步两两比较,模型对照组软骨细胞凋亡率大于正常对照组和电针治疗组(LSD-t=3.769,P=0.000;LSD-t=7.000,P=0.000),电针治疗组软骨细胞凋亡率大于正常对照组(LSD-t=10.769,P=0.000)。结论:电针治疗可以通过抑制软骨细胞凋亡,减轻膝骨关节炎软骨的损伤,这可能是电针治疗膝骨关节炎的机制之一。

Objective：To observe the effect of electroacupuncture on knee joint chondrocyte apoptosis in rabbits with knee osteoarthritis（KOA）,and to explore the mechanisms of electroacupuncture on KOA.Methods：Thirty healthy New Zealand white rabbits were randomly divided into 3 groups as normal control group,model control group and electroacupuncture group,10 cases in each group.Left knee joints of experimental rabbits in model control group and electroacupuncture group were all built models through Hulth method.At the 5th week after modeling,cases in electroacupuncture group were administrated with electroacupuncture,while the others in the normal control group and model control group were administrated with normal feeding without treatment.After 4 weeks of treatment,all of the experimental rabbits were executed for making slices of left femoral condyle which were observed on the morphous of cartilaginous tissue and chondrocyte apoptosis situation.Results：①The morphous of cartilaginous tissue：the situations as smooth cartilage surface,clearness in four-layer structure,evenly distribution of chondrocytes without clustering and complete tidemark were found in normal control group;the situations as decrease in articular cartilage thickness,rough surface,significant fibrosis and fissures,clustered chondrocytes and fractured tidemark were found in model control group;and the situations as decrease in articular cartilage thickness,unsmooth surface,rare fibrosis and fissures,clustered chondrocytes and fractured tidemark were found in electroacupuncture group.②Chondrocyte apoptosis situation：there were statistical difference in chondrocyte apoptosis rate of experimental rabbits among the 3 groups（F=126.753,P=0.000）.Chondrocyte apoptosis rate of model control group was higher than that of normal control group and electroacupuncture group respectively（LSD-t=3.769,P=0.000;LSD-t=7.000,P=0.000）,and the chondrocyte apoptosis rate of electroacupuncture group was higher than that of normal control group（LSD-t=10.769,P=0.000）.Conclusion：Electroacupuncture can relieve articular cartilage injuries of KOA through inhibiting chondrocyte apoptosis,which may be one of the mechanisms of electroacupuncture on KOA.