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Tyro 3受体酪氨酸激酶调控糖蛋白Ⅵ介导的血小板活化

Receptor Tyrosine Kinase Tyro 3 Participates in Glycoprotein Ⅵ-mediated Platelet Activation
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摘要 目的研究Tyro 3受体酪氨酸激酶在糖蛋白Ⅵ(glycoprotein VI,GPVI)介导的血小板活化中的调控作用。方法以Tyro 3基因敲除小鼠的血小板为研究对象,应用GPVI的特异性刺激剂,在体外检测血小板的聚集和铺展能力,并应用流式细胞术分析血小板的颗粒释放及整合素αIIbβ3的活化情况。结果与野生型小鼠相比,Tyro 3基因敲除小鼠血小板的聚集和铺展能力减弱,颗粒释放标记物CD 62P的数量减少,整合素αIIbβ3的活化也受到明显抑制。结论 Tyro 3受体酪氨酸激酶在糖蛋白Ⅵ介导的血小板活化中具有重要的调控作用。 Objective To study the role of Tyro 3 tyrosine kinases receptor in glycoprotein Ⅵ（GPVI）-mediated platelet activation. Methods Platelets were isolated from wild type（WT） and Tyro 3 knockout（Tyro3-/-） mice,then we examined their responses to GPVI stimulation by measuring integrin αIIbβ3 activation,granule release,aggregation and spreading. Results Compared with WT platelets,Tyro 3-deficient platelets displayed a suppressed aggregation and spreading after GPVI stimulation,a reduced CD 62P expression and integrin αIIbβ3 activition also be observed. Conclusion Tyro 3 plays an important role in GPVI,mediated platelet activation.
作者 阳艾珍 王璐 周俊松
机构地区 苏州大学医学部唐仲英血液学研究中心
出处 《血栓与止血学》 2012年第2期58-60,68,共4页 Chinese Journal of Thrombosis and Hemostasis
基金 苏州大学"211工程"建设经费项目(14121903)
关键词 Tyro3 糖蛋白Ⅵ 血小板活化 Tyro 3 Glycoprotein Ⅵ Platelet activation
分类号 Q461 [生物学—生理学]
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血栓与止血学
2012年 第2期

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