伽玛刀治疗对胰腺癌患者外周T淋巴细胞亚群的影响

Clinical analysis of immunological effects on pancreatic cancer patients by γ-knife therapy

目的研究胰腺癌患者伽玛刀治疗前后外周血白细胞、淋巴细胞及T淋巴细胞亚群的变化。方法观察20例胰腺癌患者外周血白细胞、淋巴细胞变化,并与20例健康人对照,采用流式细胞术检测伽玛刀治疗前后外周血CD4+,CD8+,CD4+/CD8+,CD4+CD25+变化。结果胰腺癌患者外周血淋巴细胞及CD4+、CD8+值低于对照组(P<0.01),伽玛刀治疗前后胰腺癌患者CD4+、CD8+含量差异具有统计学意义(P<0.05),伽玛刀治疗前后CD4+/CD8+、CD4+CD25+含量差异无统计学意义(P>0.05)。结论胰腺癌患者存在T淋巴细胞免疫功能低下,抗肿瘤免疫反应差,伽玛刀治疗使患者CD4+、CD8+T淋巴细胞进一步减少,提示伽玛刀治疗中应辅以免疫调节剂以提高临床疗效。

Objective To study the alteration in number of leucocytes,lymphocytes and the immunological function change of T cell in patients with pancreatic cancer after γ-knife therapy.Methods The peripheral blood samples were collected from 20 patients with pancreasis carcinoma and 20 healthy controls.The cell number of leucocytes and lymphocytes was studied by blood RT,the population of CD4＋,CD8＋,CD4＋/CD8＋,and CD4＋CD25＋ were evaluated by using a flow cytometry.Results The total numbers of lymphocytes were lower than those of the healthy controls.The percentages of CD4＋ and CD8＋ T cell were lower than those of the healthy controls.These differences were of statistical significance（P0.01）.After γ-knife therapy,the differences of the proportions of CD4＋ and CD8＋T cell were of statistical significance（P0.05）.The CD4＋/CD8＋ ratios and the percentages of CD4＋CD25＋ were not statistically significant（P0.05）.Conclusion Pancreasis patients have a cellular immune dysfunction and a poor anti-tumor immune response.The percentages of CD4＋ and CD8＋ T cell were lower after γ-knife therapy,which suggested that during the γ-knife therapy we should add immune regulative drug to improve therapeutic effect.