CD44表达对人肝移植急性排斥反应的诊断价值

Diagnosis value of the expression of CD44 in acute rejection after human liver transplantation

目的:探讨移植肝组织中细胞黏附分子CD44的表达对人肝移植急性排斥反应的诊断价值。方法:收集42例肝移植患者移植后肝穿刺活组织检查标本及移植前供肝标本,根据肝移植术后是否发生急性排斥反应分为急性排斥组(30例)及非急性排斥组(12例),采用免疫组织化学(免疫组化)方法检测CD44在两组移植肝组织中的表达并分析其表达与肝移植急性排斥反应的关系。结果:急性排斥组及非急性排斥组移植前供肝的CD44表达水平比较差异无统计学意义(P>0.05);急性排斥组移植后肝穿刺组织门管区及肝窦区的CD44阳性细胞百分数均明显高于非急性排斥组移植后肝穿刺组织(P均<0.05),两者与排斥反应活动指数(RAI)评分呈正相关(r=0.412,P<0.05;r=0.458,P<0.05)。结论:CD44在肝移植术后肝穿刺组织中表达增强可能与肝移植急性排斥反应的发生及其严重程度密切相关,有望成为肝移植急性排斥反应重要的辅助诊断指标。

Objective：To investigate the relationship between the expressions of CD44 and acute rejection after human liver transplantation.Methods：Fourty-two post liver transplantation biopsy tissues and pre-transplantation donor liver tissues were collected and divided into two groups,acute rejection group（n=30） and non-acute rejection group（n=12）.CD44 was detected by immunohistochemistry,and the relationship between the expression level of this marker and acute rejection was analyzed.Results：There was no significant difference of the expressions of CD44 in pre-transplantation livers of acute rejection group and non-acute rejection group（P0.05）.After transplantatiion,the expression level of CD44 in liver biopsy tissues of acute rejection group was significantly higher than that in non-acute rejection group（P0.05）.Moreover,the expression levels of CD44 in the portal areas（r=0.412,P0.05） and sinusoids（r=0.458,P0.05） were correlated positively with the degree of hepatic allograft rejection.Conclusion：Increased CD44 expression in liver graft tissue may be related with acute hepatic allograft rejection,and may be valuable biological markers of the diagnosis of acute rejection after liver transplantation.