高效液相色谱法测定双黄连有效部位中绿原酸的含量

Datermination of Chorogenic Acid Content in Effective Parts of Shuanghuanglian by RP-HPLC

目的:建立双黄连有效部位中绿原酸含量的测定方法。方法:采用Venusil XBP C18(250.0 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.05%磷酸水(2080),流速:1.0 mL.min-1,检测波长:324 nm。结果:绿原酸平均回收率为99.19%,RSD=2.82%。结论:高效液相色谱法简便、准确,可作为有效部位的质量控制方法。

Objective：To establish RP-HPLC method for determination of chorogenic acid in effective parts of Shuanghuanglian.Methods：Using Venusil XBP C18 column（250 mm×4.6 mm,5 μm） of Agela Technologies,the mobile phase was a mixture of methanol-0.05% phosphate-water（2080）.The flow rate was 1.0 mL·min-1.The wavelength was 324 nm.Results：The average recovery rate was：99.19,RSD=2.82%;Conclusion：The method is simple,accurate and can be used to control the effective parts quality.