安徽不同地区奶站牛奶中金黄色葡萄球菌及其肠毒素污染状况评估

Evaluation on the current prevalance of Staphylococcus aureus and enterotoxin pollution in dairies in different areas of Anhui province

目的评定安徽地区各奶站牛奶中金黄色葡萄球菌以及肠毒素的污染情况。方法通过从安徽省不同地区30所奶站采集乳样,进行乳源性金黄色葡萄球菌的分离与生化鉴定,并采用PCR技术对分离出的菌株进行金黄色葡萄球菌肠毒素血清型鉴定。结果安徽省30个奶站中有4个地区奶站的牛奶中污染金黄色葡萄球菌;从污染牛奶中共分离出5株金黄色葡萄球菌,检出率为16.7%。经鉴定,所分离出的金黄色葡萄球菌中2株为肠毒素A型,1株为肠毒素C型,2株为同时产肠毒素A和肠毒素C。结论安徽省不同地区奶站中的牛奶污染的金黄色葡萄球菌产肠毒素类型以肠毒素A为主。

Objective To evaluate the Staphylococcus aureus and Staphylococcal enterotoxin（SE） pollution in milk from Anhui province.Method Milk samples were collected from 30 dairies in Anhui province.Dairy-derived Staphylococcus aureus were isolated from milk samples.PCR technique was used to identify the serotype of SE.Result The milk samples from 4 dairies of Anhui province were polluted.5 strains of Staphylococcus aureus were isolated from the milk samples.The detection rate of Staphylococcus aureus was 16.7%.Upon identification,there were 2 strain of SEA positive,1 strain of SEC positive,and 2 strains of both SEA and SEC positive.Conclusion SEA contamination is the main type of Staphylococcal enterotoxin pollution in milk of Anhui province.