益生元对抗生素引起的肠道菌群失调的作用

The modulation effect of different prebiotics on intestinal flora of mice with dysbacteriosis induced by antibiotics

目的评价益生元菊糖和大蒜多糖在治疗肠道菌群失调中的作用。方法 72只Balb/c小鼠随机分为6组,随机取出12只作为正常对照组,其余灌胃盐酸林可霉素和头孢曲松钠混合抗生素溶液3 d,建立小鼠肠道失调模型后,以生理盐水灌胃作为自然恢复组,其余4组分别灌胃菊糖、低剂量大蒜多糖、高剂量大蒜多糖以及丽珠肠乐5 d。受试小鼠无菌取粪,观察各组肠道菌群的数量变化、测量血清IgG,粪便内毒素含量、有机酸含量变化。结果菊糖和大蒜多糖高剂量灌胃能加速恢复抗生素导致的小鼠肠道菌群失调,这两种益生元对内毒素的清理、免疫力的恢复也有积极的效果,效果均优于丽珠肠乐和自动恢复。结论益生元有加快肠道菌群恢复和降低抗生素的破坏效果,有益于小鼠肠道健康。

Objective To study the impact of inulin and garlic polysaccharides on intestinal flora of mice with dysbacteriosis induced by antibiotics.Method 12 mice taken randomly from 72 SPF Balb/c mice were used as controls;The remaining 60 were given gastric perfusion of lincomycin hydrochloride and ceftriaxone sodium,twice daily,for continuous 3 days to induce mouse intestinal microdysbiosis.On the fourth day,all 60 mice were randomly divided into 5 groups-inulin,high-dose garlic polysaccharides,low-dose garlic polysaccharides,Lizhuchangle and natural recovery group,and given modulating treatment with the corresponding drugs respectively for continuous 5 days;The natural recovery group was treated with saline.Result Compared with the natural recovery group,both inulin and high-dose garlic polysaccharides groups showed their abilities to promote the growth of normal intestinal flora,improve acid content,lower the endotoxin level and effectively increase IgG level（P0.05）.However,low-dose garlic polysaccharides and Lizhuchangle were less effective.Conclusion Both inulin and garlic polysaccharides at high dose have prebiotic effects on intestinal microflora imbalance induced by antibiotics in mice.