一种速干性消毒液消毒相关性能实验研究

EXPERIMENTAL STUDY ON DISINFECTION PERFORMANCE OF A KIND OF QUICK-DRYING ANTISEPTIC SOLUTION

目的研究一种速干性消毒液消毒相关性能及其影响因素。方法采用悬液定量杀菌试验和动物试验方法,对该消毒液消毒相关性能进行了实验室观察。结果该速干性消毒液为一种复方制剂,主要有效成分为8 g/L醋酸氯己定与体积分数70%乙醇。用该消毒液10倍稀释液作用2 min,对悬液内金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌的杀灭率均达到100%。菌悬液内加入有机干扰物对该消毒液杀菌作用有轻度影响,消毒液pH值7.0以上有利于杀菌效果的提高,温度越高杀菌效果越好。该消毒液急性经口毒性LD50值>5 000 mg/kg(体重),其原液对家兔完整皮肤刺激性试验最高积分为0,小鼠骨髓嗜多染红细胞微核试验结果为阴性。结论该速干性消毒液杀菌作用快速,效果较好;属于基本无毒物质,对完整皮肤无刺激性,无致微核作用。

Objective To study the disinfection performance and influential factors of a kind of quick-drying liquid skin antiseptic with chlorhexidine acetate as the active principle.Methods Suspension quantitative germicidal test and animal toxicity test were used to carry out laboratory observation of this antiseptic.Results At room temperature,the killing rate of Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa and Candida albicans exposed to the 10-fold dilution of compound chlorhexidine alcohol antiseptic solution containing chlorhexidine acetate 8 mg/L and 70%（v/v） ethanol for 2 min were 100%.Adding BSA to the bacterial suspension slightly influenced the germicidal efficacy of the antiseptic solution in killing E.coli.With the increase of pH and temperature,the germicidal efficacy of the 8-fold dilution of the antiseptic solution could obviously be enhanced in killing E.coli.The acute oral toxicity test of the disinfection solution showed that LD50 values in both rats were 5 000 mg/kg·bw,which were practically non-toxic.The skin irritation index of stock solution on rabbits was 0.The mouse bone marrow micronucleus test showed no non-nuclear-induced effect.Conclusion The quick-drying antiseptic has rapid germicidal action on vegetative forms of bacteria and natural bacteria on skin,no action of inducing micronucleus,no irritation to skin,and belongs to non-toxic substance in animal test.