体外肾系膜细胞对成骨细胞增殖及功能的影响

Effects of mesangial cell on proliferation and function of osteoblast in vitro

目的:从生长因子角度研究肾小球系膜细胞对成骨细胞增殖及功能的影响和机制。方法:应用体外细胞培养的方法,将成骨细胞分为正常血清组和系膜细胞组,分别予10%正常血清培养液及20%系膜细胞培养上清液。MTT法分别检测培养24、48、72、120 h后两组成骨细胞增殖情况;在培养72及144 h后检测上清液骨钙素(BGP)及骨桥蛋白(OPN)浓度;在培养144 h后检测培养液胰岛素样生长因子-α(IGF-α)、转化生长因子-β(TGF-β)的浓度。结果:在培养的各个时间点,系膜细胞组的成骨细胞增殖均明显高于正常血清组(P<0.05)。在培养72、144 h后,系膜细胞组BGP及OPN的浓度分别高于正常血清组11.3%、16.4%和55.0%、39.6%。在培养144 h后,系膜细胞组的IGF-α、TGF-β浓度比正常血清组分别增高10.1%和47.7%。结论:肾小球系膜细胞能直接作用于成骨细胞,促进成骨细胞的增殖及功能。这种作用可能是通过促进成骨细胞分泌TGF-β来实现的。

Objective：To explore the effects and mechanism of mesangial cell（MC） on proliferation and function of osteoblast in vitro from growth factors.Methods：Osteoblast cultured in vitro were divided into normal blood serum group and MC group and cultured supernatant fluid by 10% serum culture medium and 20% MC culture medium.MTT was used to checked the proliferation of osteoblast at the end of 24,48,72,120 h;Expression of BGP and OPN were tested 72 h and 144 h after culture;Expression of IGF-α and TGF-β were tested 144 h after culture.Results：The proliferation of osteoblast in MC group was obviously higher than normal blood serum group in different times（P0.05）;The level of BGP and OPN of osteoblast was obviously higher than normal blood serum group（11.3%,16.4%,55.0% and 39.6%）;and the level of IGF-α and TGF-β of osteoblast was obviously higher than normal blood serum group（10.1% and 47.7%）.Conclusion：MC can directly act on osteoblast,promote the proliferation and function of osteoblast by promoting secretion of TGF-β.