针刺后猫大脑皮层不同温区ATP酶活性及P物质含量比较

Comparison of Activities of ATPase and Contents of SP in Different Temperature Areas of Cat's Cerebral Cortex after Acupuncture

本实验选用家猫 ,在红外热像仪监视下 ,选取针刺后皮层温度较高和较低部位的组织进行分析 ,用酶学方法测定Na+,K+ ATP酶活力 ,放射免疫方法 (RIA)测定P物质 (SP)含量。实验结果如下 :针刺后 ,皮层温度较高部位 (3 4.9± 1 .1 3℃ )的Na+,K+ ATP酶活力与皮层温度较低部位 (3 3 .3 8± 1 .0 3℃ )比较 ,有明显升高 (P <0 .0 1 ) ;皮层温度较高部位SP含量亦较温度低部位显著升高 (P <0 .0 2 )。结果提示 ,针刺后大脑皮层某些区域温度的高低可能与中枢Na+,K+ ATP酶活力增强有关 。

Domestic cats were used in this experiment. After acupuncture, the higher temperature area and lower temperature area of the cerebral cortex were dissected respectively under the monitoring of the infra red thermography. The activities of the Na +,K + ATPase in the cerebral cortex were determined with enzyme method and the contents of SP (substance P) were determined with radioimmunoassay. The results show that the activities of Na +, K + ATPase in the higher temperature area (34.90±1.13 ℃) is higher than that in the lower temperature area (33.38±1.03 ℃), P<0.01;the contents of SP in the higher temperature area is also higher than that in the lower temperature area (P<0.02) after acupuncture. It suggests that the change of temperature caused by acupuncture at some parts of the cerebral cortex is related to the activities of Na + ,K + ATPase and the contents of SP in the central nervous system of the cat.