葡萄籽原花青素增强X射线辐射敏感性的实验研究

Study on the Effect of Grape Seed Proanthocyanidins on Increasing the Roadiosensitivity for X-ray

目的:研究葡萄籽原花青素(GSPs)对人肝癌HepG2细胞、人宫颈癌Hela细胞和人白血病K562细胞的X射线增敏作用,并探讨其作用机制。方法:应用SRB法和集落形成法测定GSPs对三株细胞的X射线增敏作用,单因素方差分析确定各因素对细胞杀伤的贡献,单击多靶模型曲线拟合计算增敏比。结果:SRB检测结果表明,GSPs可以抑制三株细胞的增殖,呈现时间和剂量依赖效应;但对不同细胞的增殖抑制作用差异较大,对人白血病K562细胞的抑制作用最强,对人肝癌HepG2细胞、人宫颈癌Hela细胞的抑制作用较弱;对X射线增敏作用结果表明,GSPs对人白血病K562细胞的增敏作用最强,最佳增敏剂量为6.25～12.5μg/mL,对人肝癌HepG2和人宫颈癌Hela细胞的增敏作用相似,最佳增敏剂量为12.5～25μg/mL;集落形成法检测结果表明,应用增敏剂量的GSPs后,对人白血病K562细胞表现出较好的辐射增敏作用,平均致死剂量D0值和准阈剂量Dq减小,细胞对X射线辐射敏感性增加,亚致死损伤修复减少,增敏比为1.94。结论:GSPs可以增强肿瘤细胞对X射线的敏感性,最佳增敏剂量为12.5～25μg/mL,其机理可能通过抗氧化作用,调节细胞细胞内氧平衡,诱导细胞产生G1期阻滞而发挥辐射增敏作用。

Objective：To investigate the effect of Grape Seed Proanthocyanidins（GSPs） on enhancing the radiosensitivity of human hepatic carcinoma cell line HepG2,human cervical cancer cell line Hela and human leukemia cell line K562 for X-ray in vitro.Methods：The killing effect of GSPs combined with X-ray on cells was evaluated by SRB and clone formation assay.Results：The GSPs had obvious cytotoxicity on three cell lines in a dose-dependent and time-dependent manners.However,inhibition rate of different cell line were quite different,the strongest one was human leukemia K562 cells and the others were weak.The sensitization ranges calculated by univariate analysis were 6.25～12.5μg/mL for human leukemia K562 cells.Sensitization enhancement ratio was 1.94 using curve fitting method for K562 cells.Conclusion：GSPs can obviously enhance the radiosensitivity of cancer cells in vitro.The mechanism of sensitization effect may be related to the effects of GSPs on oxygen balance and cell cycle.