杜仲叶桃叶珊瑚苷的酶法提取及其抑菌活性

Enzymatic Extraction and Antibacterial Activity of Aucubin from Eucommia ulmoides Leaves

目的:研究杜仲叶桃叶珊瑚苷的酶法提取工艺及其抑菌活性。方法:采用纤维素酶法,以桃叶珊瑚苷提取率为指标,优选最佳提取条件,并对其抑菌效果进行研究。结果:杜仲叶桃叶珊瑚苷的最佳提取工艺为:料液比1∶12,酶解pH 6.0,酶用量0.4%,酶解温度50℃,酶解时间50 min,溶出量可达17.892 mg/g。桃叶珊瑚苷提取液对大肠杆菌和金黄色葡萄球菌的抑菌作用较强,对金黄色葡萄球菌的MIC为4.832 mg/mL,对大肠杆菌的MIC为9.664 mg/mL,但对肺炎链球菌和MG溶血性链球菌的抑菌作用不明显,MIC均为28.946 mg/mL。结论:该提取工艺合理可行,提取率高,提取得到的桃叶珊瑚苷具有一定的抑菌作用。

Objective：To investigate the technology of Aucubin in Eucommia ulmoides leaves extracted by enzymatic method and its antibacterial activity.Methods：Aucubin in Eucommia ulmoides leaves was extracted by cellulase method.The extraction technology was optimized using the content of Aucubin as index.The antibacterial activity of Aucubin was determined.Results：The results showed that the optimum technology was as follows;The solid-liquid ratio was 1∶12,extracted for 50 min by 0.4% enzyme at 50 ℃ in pH 6.0.The extraction rate of Aucubin was as high as 17.892 mg/g.The Aucubin extracted could obviously inhibit the growth of Escherichia coli and Staphylococcus aureus,the MIC of Aucubin against Staphylococcus aureus and Escherichia coli were 4.832 mg/mL and 9.664 mg/mL respectively.However,Aucubin presented weak inhibitory effect on Streptococcus pneumonia and MG-hemolytic streptococcus,the MIC of Aucubin against them were all 28.946 mg/mL.Conclusion：The extraction technology obtained in this experiment is reasonable and feasible with high extraction rate,and the Aucubin has some antibacterial activity.