哮喘动物模型气道中白细胞介素5和10的动态变化及其意义

A study on IL-5 and IL-10 in the modulation of asthmatic airway inflammation in murine models

目的 研究炎症促进因子白细胞介素５（ＩＬ－５）和炎症抑制因子ＩＬ－１０的变化趋势、相互关系和对炎症反应的调节作用。方法 卵白蛋白腹腔注射与雾化吸入诱发ＢＡＬＢ／ｃ小鼠哮喘发作，动态观察哮喘动物气道炎症的改变。在激发后０、８、２４、４８、９６ｈ及７、１４ｄ行支所管肺泡灌洗，测定回收液中细胞总数与分类计数，酶联免疫吸附（ＥＬＩＳＡ）法测定ＩＬ－５，ＩＬ－１０浓度。结果

] Objective to observe the time-course profile of airway inflammation in murine sathma model,and to analyze the dynamic prodile of pro-inflammtory factor IL-5,and inflammtion-suppressing factor IL-10 in the airway .Methods An animal model of asthma was established by OVA sensitzing-challenging BALB/C mice.At seve points (Oh,8h,24h,48h,96h, dsys 7 and days 14) in the time course after challenge , bronchoalveolar lavage (BAL) was performed.Total cell counts, cell diffrential, eosinophil(EOS) counts and peroentags were examined and levels of two Th2 cytokines,IL-5 and IL-10, were detected by ELISA. Results After OVA challenge, total cell counts, EOS counts and EOS pereentages in BALF were increased significantly.EOS count increased at 8h, formed a plateau from 24h to 48h, declined at 96h, reduced approximately to control from days7 to days14.IL-5 in BALF elevated at 8h, maitained at high level for 28h to 96h, decreased at days7.The dynamic changes of IL-5 was in synchronism with that of EOS counts, a positive correlation being identified NO significant difference was found between IL-10 in BALF before and after challenge.A clase correlation between EOS counts and IL-5/IL-10 ration was demonstrated (r = 0.9, P < 0.001). Conclusion IL-5 and IL-10 and downregulate asthmatic airway inflammation respectively,and their interaction may play an important role in inflammatory progress and resolution.[