摘要
牛磺酸可能是中枢神经系统中一种抑制性神经递质或神经调质。本实验目的在于观察牛磺酸是否引起背根神 经节(DRG)神经元膜产生反应以及是否对GABA引起的背根神经节神经元膜电流有调制作用。应用全细胞膜片钳 技术,观察未检测到牛磺酸(10~-6~10~-4mol/L)对DRG神经元膜电流的改变,但预先给牛磺酸对GABA(10~-6~10~-4mol/ L)激活的膜内向电流有明显的抑制作用,并具明显的浓度依赖性。如 10~-5mol/L的牛磺酸可使 10~-5mol/L GABA激活 的膜电流抑制(40%±5.9%,n=5)。牛磺酸对GABA激活膜电流的抑制作用随预加牛磺酸与给予GABA之间间隔时 间的延长而逐渐减弱,间隔8分钟左右,GABA激活电流完全恢复。结果提示牛磺酸可能作为神经调质在初级感觉信 息传递中发挥重要的调制作用。
Taurine has been widely postulated to play a specific role as an-inhibitory neurotransmitter or neuromodulator. This study aimed to explore whether taurine could induce response of the membrane and whether GABA-activated current be mediated by taurine using whole-cell patch-clamp technique. The application of taruine (10^-6-10^-4mol/L) failed to change the membrane current in fat dorsal root ganglion neurones, but taurine was found to exert negative modulatory effects on GABA-activated current. In our experiment, GABA (10^-6- 10^-4mol/L) -activated current were attenuated markedly and reversibly by pre-application of taurine. The inhibitory effect of taurine (10`-6~ 10^-4mol/ L) on GABA (10^-6~ 10^-4mol/L ) -activated current was concentration dependent. The more the interval between pretreatment with taurine and the application of GABA was lengthened, the less inhibitory effect of taurine on GABA activated current would be. It took about 8 min to get a full recovery from taurine inhibition. Our observation suggested that taurine could be considered a good condidated for the endogenous neuromodulator on the primary afferent terminal of rat spinal cord.
出处
《基础医学与临床》
CSCD
2000年第2期61-64,共4页
Basic and Clinical Medicine
基金
国家自然科学基金!(No.39860027)
石河子大学课题资助
关键词
牛磺酸
Γ-氨基丁酸
背根神经节
GABA
taurine
GABA: dorsal root ganglion: whole-cell patch-clamp: modulation
rat