谷氨酸对大鼠海马CA_1区锥体细胞电压依赖性Na^+电流的影响

Effect of glutamate on voltage-dependent Na^+ currents of acutely dissociated Hippocampal CA_1 pyramidal cells in rat

本实验采用全细胞膜片钳技术，观察了谷氨酸对急性分离的海马ＣＡ_１区神经元的电压依赖性ＮＡ~＋通道的影响， 结果发现谷氨酸对电压依赖性Ｎａ~＋电流有明显抑制作用，其抑制方式呈电压依赖性、浓度依赖性和时间依赖性，表明 谷氨酸对电压依赖性钠通道有抑制作用。

The effect of glutamate on voltge-dependent Na^+ current in acutely isolated hippocampal CA_1 pyramidal neurons of postnatal rats (7~ 10days ) were studied by means of the whole cell recording patch clamp technique. The results realed that glutamate induced an apparent blockage of INa. Its blockage on INa has voltage-dependent, dose-dependent and timedependent characteristics. These results suggested that glutamate can inhibit voltage-dependent Na^+ Channel.