采用基因释放剂对微量及陈旧标本进行直接PCR扩增

Direct PCR amplification from samples of microliters/micrograms and long term storage by using gene releaser

为简化ＰＣＲ方法和减少标本用量，利用基因释放剂不提取ＤＮＡ，比较了利用基因释放剂和不用基因 释放剂，对微量肝素抗凝血、微量结肠粘膜组织（置－２０℃６个月）和含有ＰＣＲ抑制剂的ＤＮＡ标本直接进 行ＰＣＲ扩增。结果，利用基因释放剂，可扩增出目的产物，不用基因释放剂，来扩增出目的产物。提示利用 基因释放剂直接进行ＰＣＲ，简单快速，不用提取ＤＮＡ，适于微量、含有ＰＣＲ抑制物的ＤＮＡ及陈旧组织标本 的检测。

In order to simplify PCR without extraction of DNA,and to detect the samples with PCR inhibitors,and to minimize the size of samples to a few microliters/micrograms,gene releaser was used. Heparinized blood samples of microliters,colonic mucosa tissues (stored in --20C over 6 months ) of micrograms and DNA samples with PCR inhibitors were checked with direct PCR with and without (as control ) gene releaser. PCR amplification product was positive with gene releaser,but negative without. Gene releaser is a very useful agent for the rapid and simplified direct PCR amplification without extraction of DNA,and suitable for detection of samples of microliters/micrograms and DNA with PCR inhibitors as well as tile long time stored tissues.