喉鳞癌中iNOS及CD105的表达及其与血管生成的相关研究

Expressions of inducible nitric oxide synthase and CD105 in laryngeal squamous cell carcinoma and their relations with angiogenesis

目的探讨诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)及CD105在喉鳞癌组织中的表达及其临床病理意义。方法应用免疫组化SP法检测iNOS在40例喉癌标本及10例正常喉黏膜标本中的表达,并计数CD105标记的微血管密度(microvessel density,MVD)。结果 iNOS在喉癌组织阳性表达明显增高,与正常喉黏膜组织比较有统计学意义(P<0.01),iNOS蛋白表达与喉癌患者颈部淋巴结转移、肿瘤分化及临床分期有关(P<0.05),而与肿瘤的临床分型以及患者的年龄、性别等因素无关(P>0.05),40例喉癌组织中MVD值[(23.45±8.27)个/HPF]显著高于正常喉黏膜组织(P<0.01),并且与iNOS表达正相关(P<0.05)。结论 iNOS与喉鳞癌的侵袭及转移相关,喉鳞癌组织MVD增高与iNOS有关,iNOS可能通过促进新生血管形成影响喉鳞癌的发生、发展。

Objective To study the expressions of inducible nitric oxide synthase（iNOS） and CD105 in laryngeal squamous cell carcinoma（LSCC） and their clinical significance.Methods The immunohistochemical SP method was used to examine the expressions of iNOS and CD105 in 40 cases of LSCC and 10 cases of normal mucosae,and the microvessel density（MVD） highlighted by CD105 were counted.Results iNOS expression in tumour tissue was significantly higher than normal mucosa（P0.01）,which was related with the metastasis of cervical glands,TNM stage and pathology grading of laryngeal cancer（P0.05）,but no relation with gender,age and tumor site（P0.05）.MVD highlighted by CD105 in tumour tissue [（23.45±8.27）/HPF] was significantly higher than normal mucosa（P0.01）.The degree of iNOS expression and MVD in tumor showed positive correlation（P0.05）.Conclusion Higher expression of iNOS is related to the invasion and metastasis of LSCC,The high MVD in LSCC may be related with the expression of iNOS.iNOS may facilitate the pathogenesis and development of LSCC via accelerating the angiogenesis of the tumor.