摘要
将20只SD雌性大鼠随机分为模型组和对照组,模型组(n=12只)喂高糖高脂饲料,对照组(n=8只)喂基础饲料。大鼠喂养4周后,以小剂量链脲佐菌素(STZ,35 mg/kg)腹腔注射,建立2型糖尿病大鼠模型。结果表明:与对照组相比,模型组血清胰岛素和空腹血糖明显上升(p<0.01),胰岛细胞数目减少,细胞质变淡。高脂喂养联合STZ(35 mg/kg)诱导大鼠2型糖尿病的方法有效且造模长期稳定、成功率高等特点,是人类2型糖尿病及其药物研究的理想动物模型。
Twenty SD female rats were randomly divided into two gi＇oups, control group and model group. In model group （n = 12）, the rats were fed with high-fat and high-sugar pellet, and in control group （n = 8）, the rats were fed with commercial standard pellet. Four weeks after feeding, intraperitoneal injection of low dosage of streptozotocin （STZ, 35 mg/kg） was conducted to rats in both groups in order to build type 2 diabetic rat models. The resuhs indicated that in comparison with control, the serum insulin levels and the fasting blood glucose （FBG） in model group all increased significantly （P〈0.01）, the [3-cell number decreased and the cytoplasm became light. The method that inducing type 2 diabetic rat by combining high-fat and high-sugar feeding with intraperitoneal injection of STZ with 35 mg/kg is effective, and the models built by the method are stable for a long period of time and with high success rate. Therefore, the models built by the method are the ideal animal model for studying human type 2 diabetes mellitus and its drug.
出处
《湖南农业科学》
2012年第4期130-132,共3页
Hunan Agricultural Sciences
基金
湖南省优秀研究生科研创新项目(CX2011B293)
