p38信号通路在脂多糖诱导的大鼠系膜细胞产生白细胞介素1β中的作用

The role of p38MAPK in the production of inflammatory cytokines in rat glomerular mesangial cells caused by LPS

目的探讨p38信号通路在肾小球系膜细胞促炎介质白细胞介素1（IL-1）β表达中的作用。方法采用免疫沉淀法、免疫复合物蛋白激酶测定，Westernblotting检测p38信号通路在脂多糖（LPS）诱导的肾小球系膜细胞炎症反应中的活化程度，应用逆转录-聚合酶链反应（RT－PCR）和ELISA观察p38信号通路抑制剂SB203580对肾小球系膜细胞促炎介质IL－1βmRNA和蛋白质表达的影响。结果脂多糖刺激肾小球系膜细胞引起p38信号通路活化，p38信号通路抑制剂SB203580对肾小球系膜细胞促炎介质表达有显著抑制作用。结论p38信号通路在大鼠系膜细胞炎症反应产生炎症介质IL－1β中可能起主要的作用。

Objective To investigate the action and mechanism of p38 mitogen-activated protein kinase(p38MAPK) in inducing inflammatory cytokines in rat glomerular mesangial cells caused by LPS. Method Immunoprecipitation, immune complex protein kinase assay and Westem blotting were used to detect p38MAPK in rat glomerular mesangial cells caused by LPS. RT-PCR and EUSA were used to investigate the effect of SB203580 on transcription of IL-1β mRNA and translation of protein in mesangial cells. Results LPS caused activation of p38MAPK at time and dosage dependent manner, and SB203580inhibited not only activation of p38MAPK but also production of proinflammatory cytokines at time and dosage dependent manner. Conclusion p38MAPK may play an important role in production of proinflammatory factors in the rat glomerular mesangial cells.