摘要
目的观察槲皮素对SW480细胞增殖和凋亡的影响并探讨其机制。方法采用流式细胞检测技术检测槲皮素诱导人结肠癌SW480细胞的凋亡能力;应用免疫细胞化学技术检测细胞中己糖激酶蛋白的表达。结果槲皮素以浓度依赖性的方式诱导结肠癌SW480细胞的凋亡并下调己糖激酶蛋白的表达。结论槲皮素通过抑制己糖激酶的表达来促进结肠癌SW480细胞凋亡。
Objective Investigate the apoptogenic activities of quercetin on SW480 cells.Methods Annexin V-FITC/PI stained fluorescence-activated cell sorter（FACS） was used to detcted the apotosis rate,Immunocytochemistry was used to examine cellular localisation and hexokinase protein levels.Results quercetin produced dose-dependent inhibition of SW480 cell growth and induced its apoptosis,the expressions of hexokinase proteins were down regulated by quercetin.Conclusion The quercetin induce SW480 cells apoptosis through downregulation of hexokinase
出处
《中国实用医药》
2012年第8期58-59,共2页
China Practical Medicine
关键词
结肠癌
槲皮素
凋亡
己糖激酶
Colon carcinoma
Quercetin
Apoptosis
Hexokinase
