缩宫素对人骨肉瘤细胞系MG-63增殖及成骨活性的影响

Influence of oxytocin in proliferation and osteogenesis activity of MG-63 cells

目的:研究缩宫素(OT)对人成骨细胞系MG-63增殖及成骨活性的影响,阐明其在骨代谢调节方面的作用。方法:用含OT 10、50和100 nmol.L-1的培养基孵育MG-63细胞1、3、5和7 d采用MTT法检测OT作用下细胞增殖活性的变化,选择其中最低有效浓度进一步检测OT作用下MG-63碱性磷酸酶(ALP)活性的变化。结果:MTT法检测显示,50和100 nmol.L-1OT作用1、3、5和7 d后MG-63细胞的增殖活性明显高于对照组(P<0.05);10 nmol.L-1作用7 d后MG-63细胞增殖活性明显高于对照组(P<0.05)。ELISA检测显示,50 nmol.L-1OT作用3、5和7 d后MG-63细胞ALP活性明显高于对照组(P<0.05)。结论:OT对MG-63细胞增殖有调节作用,能够正向促进成骨细胞的增殖和分化。

Objective To study the influence of oxytocin（OT） in the proliferation and osteogenesis activity of MG-63 cells,and to clarify its function on bone metabolism.Methods The MG-63 cells were incubated with 10,50 and 100 nmol·L^-1OT for 1,3,5 and 7 d.The cell proliferation was detected by MTT,then the minimum effective concentration was used to detect the change of alkaline phosphatase（ATP） activity .Results The MTT test results showed that the proliferation of MG-63 cells after treated with 50,100 nmol·L^-1OT for 1,3,5 and 7 d was significantly higher than those in control group（P〈0.05）;and the proliferation of MG-63 cells after treated with 10 nmol·L^-1OT for 7 d was significantly higher than that in control group（P〈0.05）.The ELISA test results showed that the ALP activities of MG-63 cells after treated with 50 nmol·L^-1OT for 3,5 and 7 d were sifnificantly higher than those in control group（P〈0.05）.Conclusion OT has a regulation effect on proliferation of MG-63 cells,and it can promote the proliferation and differiation of MG-63 cells.