RP-HPLC法测定心宁片中丹参素和原儿茶醛的含量

Determination of Tanshinol and Protocatechuic Aldehyde in Xinning Tablets by RP-HPLC

目的建立同时测定心宁片中丹参素和原儿茶醛含量的方法。方法采用高效液相色谱法,色谱柱为LinkSil ODS C18;柱温:35℃;流动相:甲醇-1%冰醋酸溶液(8∶92);流速:1.0mL.min-1;检测波长:280nm。结果丹参素在23.4～187.2mg.L-1(r=0.999 8)、原儿茶醛在1.92～38.40mg.L-1(r=0.999 9)浓度范围内线性关系均良好;平均回收率分别为100.29%和98.96%。结论该方法简单、准确、快速,可用于心宁片中丹参素和原儿茶醛的质量控制。

Objective To establish a method for simultaneous determination of tanshinol and protocatechuic aldehyde in Xinning Tablets.Methods An HPLC method was carried out.A LinkSil ODS C18 column was used with methanol-1% glacial acetic acid（8∶92）as the mobile phase at the flow rate of 1.0 mL·min-1.The detection wavelength was 280 nm and the column temperature was 35℃.Results The linearity was obtained in the range of 23.4～187.2 mg·L-1（r=0.999 8）for tanshinol and 1.92～38.40 mg·L-1（r=0.999 9）for protocatechuic aldehyde,respectively.The average recoveries of tanshinol and protocatechuic aldehyde in Xinning Tablets were 100.29% and 98.96%.Conclusion The method is simple,accurate and fast,which is suitable for the quality control of tanshinol and protocatechuic aldehyde in Xinning Tablets.