无泵负压进样-芯片电泳分离-激光诱导荧光光谱法测定儿茶酚胺类物质

Separation and Determination of Catecholamines by Microchip Electrophoresis with Laser Induced Fluorescence Spectrometry and Pump-free Negative Pressure Sample Introduction

提出了用芯片电泳分离-激光诱导荧光光谱法测定儿茶酚胺类物质的方法。采用自制的无泵负压进样系统,避免了进样歧视效应。在优化的条件下,去甲肾上腺素(NE)、多巴胺(DA)和肾上腺素(E)可在1 min内完全分离。3种儿茶酚胺的平均迁移时间依次为30.59,37.23,46.43 s,其相对标准偏差(n=7)依次为1.10%,1.28%,0.45%。3种物质的线性范围为0.3～5.0 mg.L-1(NE及DA)和0.05～4.0 mg.L-1(E),检出限(3S/N)依次为30,30,10μg.L-1。

The method of microchip electrophoresis with laser induced fluorescence detection was proposed to be used in the separation and determination of catecholamines, including norepinephrine （NE）, dopamine （DA） and epinephrine （E）. The application of a self-made pump-free negative pressure sample introduction system made it possible to avoid the discrimination effect in sample introduction. NE, DA and E were separated completely within 1 min under the optimum working conditions, with their average migration times of 30. 59, 37. 23, 46. 43 s and values of RSD＇s （n=7） of 1.10%, 1.28% 0. 45% respectively. Linearity ranges found were 0. 3-5.0 mg · L^-1 （for NE and DA） and 0. 05-4. 0 mg · L ^-1 （for E）, with detection limits （3S/N） of 30, 30, 10μg · L^-1 respectively.