d-筒箭毒碱对V79细胞和小鼠骨髓细胞有丝分裂的影响

STUDY OF MITOTIC EFFECTS OF d TUBOCURARINE IN V_(79) CHINESE HAMSTER CELLS AND IN MOUSE BONE MARROW CELLS

目的 :探讨烟碱型乙酰胆碱受体阻断剂d 筒箭毒碱对V79细胞和小鼠骨髓细胞有丝分裂的影响。方法 :在离体情况下以受试物处理中国仓鼠V79细胞 ,通过分析V79细胞晚末期和早G1期细胞核与细胞质构型、双核细胞频率 ,探讨受试物对细胞质质裂的影响 ;研究同时探讨了活体情况下 ,该化合物对小鼠骨髓细胞的C 有丝分裂 (C———M)效应 ,分析其对哺乳动物体细胞有丝分裂的影响。结果 :d 筒箭毒碱能使V79细胞的晚末期—早G1期细胞核和细胞质分裂构型发生显著变化 (P <0 .0 0 1)、双核细胞的频率显著提高 (P <0 .0 0 1)。在小鼠骨髓细胞C———M效应分析中 ,该化合物除在低剂量组导致较明显的C—有丝分裂细胞增加外 (P <0 .0 5 ) ,其他未见异常。结论 :研究提示受试物可通过N型胆碱受体阻断过程对哺乳动物细胞质裂的真实性产生影响 ;同时 ,该化合物还具有影响染色体正常分离的潜在能力。对胆碱受体功能的研究将有助于探索非整倍体产生的各种机制。

Purpose: The mitotic effects of d tubocurarine in V\-\{79\} cells and mouse bone marrow cells was investigated in this study. d tubocurarine is a antagonist to cholinergic receptors and inhibitor of nicotinic receptor. Methods: V\-\{79\} Chinese hamster cells were treated in vitro by d tubocurarine. The alteration of late telophase early G1 configuration induced by this chemical was determined by analyzing of presence or lack of a cleavage furrow in late telophase and very early G1 cells, the abnormal cytokinesis was investigated for the frequencies of binuclear cells in interphase cells. Simultaneously, we investigated the C M effects of d tubocurarine in mouse bone marrow cells. Results: The tested chemical disturbed mitotic coordination and significantly induced high frequency of binuclear cells in V\-\{79\} cell line (P<0.001). There is no other distinguished abnormality in mouse bone marrow cells but significant enhance of C M cells at low dose group. Conclusion: The results indicated that d tubocurarine is able to inhibit mammal mitosis by blocking the function of nicotinic receptor. The malfunction of cholinergic and nicotinic receptors may be a mechanism of aneuploid induction.