摘要
目的 :为研究油烟凝聚物与肿瘤发生的潜在关系及大蒜油的防护作用。方法 :用油烟凝聚物和大蒜油分别或同时处理V79细胞 ,利用免疫细胞化学方法检测V79细胞中 p5 3、p2 1ras蛋白的表达情况。结果 :与对照组相比 ,油烟凝聚物处理组 p5 3、p2 1ras蛋白表达明显升高 (P <0 .0 1) ,且有剂量———效应关系 ;与对照组相比 ,5 0 μl/ml大蒜油处理组降低油烟凝聚物诱发V79细胞 p5 3、p2 1ras蛋白表达水平 (P <0 .0 5或P <0 .0 1)。结论 :油烟凝聚物有诱变作用 ,大蒜油可防护油烟凝聚物的诱变作用。
Purpose: This paper studies the relationships between Heated Cooking Oil Vapors(HCOV) and Allium Sativum Oil(ASO) in mutagenesis. Methods: we detected p53 and p21\+\{ras\} protein expression in the V\-\{79\} cells treated by HCOV and ASO with immunocytochemical techneques. Results: The results showed that the p53 and p21\+\{ras\} protein expression was significantly increased in HCOV groups compared with the control group(P<0.01) , and a dose dependent relationship was observed, while the ASO(50μg/ml) could decreased the p53 and p21\+\{ras\} protein expression in the V\-\{79\} cells treated by HCOV compared with the control group (P<0.05 or P<0.01). Conclusion: The results indicated that the ASO could prevent from mutagenic activity of the HCOV.
出处
《癌变．畸变．突变》
CAS
CSCD
2000年第1期42-44,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
油烟凝聚物
大蒜油
P53
肿瘤发生
抗肿瘤作用
heated cooking oil vapors
ailium sativum oil
p53
p21\+\{ras\}
immunocytochemistry
