乙肝病毒携带产妇血浆与初乳中病毒含量的相关性研究

Correlation analysis between plasma and colostrum viral load in hepatitis B virus carrier puerperae

目的探讨乙肝病毒携带产妇血浆及初乳中病毒含量的差异性及其临床意义。方法选取20例乙肝病毒携带产妇作为实验组，21例乙肝表面抗原阴性的健康产妇作为对照组，采用RT—PCR法检测实验组及对照组血浆及初乳中HBVDNA病毒含量。结果对照组产妇的初乳和血液中HBVDNA均检测阴性。实验组产妇初乳中HBVDNA含量与血浆中HBVDNA含量有一定相关性，ct值和病毒载量相关系数r分别为0．7731和0．8053，P值均小于0．0001。实验组血浆中HBVDNA含量明显高于初乳中HBVDNA含量10～1000倍（P〈0．0001）。当血浆HBVDNA国际单位（IU）的Log值下降到4．7以下时，75％的乙肝病毒携带产妇初乳中病毒DNA是低于检测限。结论血浆和乳汁中HBVDNA载量较低的乙肝病毒携带产妇可结合其它临床检测指标部分考虑采取母乳喂养。

Objective To investigate the correlation between plasma and colostrum viral load in carrier puerperae and evaluate its clinical significance. Methods 20 cases of hepatitis B virus carrier puerperae were enrolled as the experimental group, 21 cases of hepatitis B surface antigen-negative healthy puerperae were selected as a control group. HBV DNA was detected by RT-PCR. Results HBV DNA was below the lowest detection limit both in colostrum and plasma in control group. In HBV carrier group, a statistically significant direct correlation was observed（ r = 0. 8053, P 〈 0. 0001 ） between colostrum and plasma HBV viral load. The concentration of HBV DNA in plasma was 10 to 1000 fold （ P 〈 0. 0001 ） higher than that in colostrum. When plasma HBV DNA load in the Log value dropped to below 4. 7 IU, colostrum viral DNA of 75 % hepatitis B virus carrier puerperae was below the detection limitation. Conclusion The puerperae with low levels of HBV DNA in colostrum and plasma could be considered to applied breastfeeding.