百合知母汤对哮喘大鼠血清、肺泡灌洗液中SP-A及脏器系数变化的影响

Research of the Effection of Baihe-Zhimu Decoction on Asthmatic Mouse Serum,IgE in Alveolar Lavage Fluid and Lung Tissue Changes

目的:通过百合知母汤对哮喘大鼠的干预,探讨该治疗方法的作用机理。方法:将大鼠随机分成正常组、哮喘模型组、中药大、中、小剂量组、地米组、中西药联用组,除正常组外经卵蛋白免疫建立模型,ELISA法检测各组血清和BALF中SP-A的含量;计算肺脏、胸腺和脾脏的脏器系数。结果:与正常组相比,模型组及小剂量组血清、BALF中SP-A明显降低(P<0.05);与模型组比较,中药各剂量组、地米组及中西药联用组血清和BALF中SP-A有统计学意义(P<0.05);中药各剂量组间比较,中剂量组血清和BALF中SP-A均高于小剂量组,差异有统计学意(P<0.05);模型组肺脏器系数明显高于正常对照组(P<0.05)。结论:百合知母汤可显著升高血清和BALF中SP-A含量,对肺组织有很大影响。

Objective： Through the Baihe-zhimu decoction intervention,the effection of this treatment method on asthmatic mouse and lung tissue is researched.Methods： Devide the mice into the normal group,asthma group,Chinese medicine high dose,middle dose,and low dose groups,dexamethasone group,and combine of western and Chinese medicine group.Except the normal group,establish the model by ovalbumin immunized,detect the SP-A in BALF and serum,the ratio of lung thymus and spleen and body weight were calculated.Results： Compared with the normal group,the sera,SP-A in BALF and serum,the asthma group and low dose group reduce significantly（P〈0.05）.Compared with the asthma group,the sera,SP-A in BALF and serum,three Chinese medicine groups,DXM group and combination group have statistical meaning（P〈0.05）.Between the three Chinese medicine groups,the sera,SP-A in BALF and serum,middle dose are less than low dose groups,and their differences have statistical meaning（P〈0.05）.The ratio of lung and body weight in the asthma group rise significantly than the normal group（P〈0.05）.Conclusions： Baihe-zhimu decoction can rise the SP-A in BALF and serum significantly lung may be influenced primarily during the airway remodeling of chronic asthma.