前列腺癌细胞Transwell小室体外侵袭模型的建立及应用价值研究

Establishing Transwell Chamber for Prostatic Carcinoma Cell Study

目的:建立前列腺癌细胞transwell小室体外侵袭模型,并探讨该侵袭模型在前列腺癌转移研究方面的意义。方法:将200μl2组前列腺癌细胞加入Transwell小室侵袭模型上室分别培养12、24、36、48h,加入侵袭模型上室的细胞取不同浓度(1.0×105、2.0×105、3.0×105、4.0×105/m1)温箱中培养,在不同时间点观察不同浓度的前列腺癌细胞穿过基质膜的细胞数,测定2组前列腺癌细胞系的侵袭能力。结果:穿过ECM胶聚碳酸酯膜的细胞在第12h较少,而随着时间的延长逐渐增多。细胞穿膜在24小时前后最显著;同时,穿过人工基底膜的细胞数随着细胞浓度增高而增多,但细胞浓度大于3.0×105/ml时,穿过人工基底膜的细胞数无明显变化。结论:本实验成功建立了前列腺癌细胞Transwell小室体外侵袭模型,其在前列腺癌研究方面具有较高的应用价值。该侵袭模型的建立可间接判断肿瘤的侵袭力并且对探明前列腺癌的转移机制及影响因素具有重要意义。

Objective： To establish a transwell chamber for invasive human prostatic carcinoma cell.Methods： Transwell chamber in vitro assay was used to invasion of two cell lines.200μl two cell of various concentrations（1.0×10^5,2.0×10^5,3.0×10^5,4.0×10^5/ml） was added into the upper chamber,and the cells were allowed to penetrate the membrane for 12,24,36 and 48,respectively.The invasive rate was asesseed by quantifying cells on the under surface of the membrane.Results： The invasion of two prostatic cell lines was different.The invasive cells were low 12 hours after the incubation,and which increased dramatically at 24 hours.24 hours later the rate reached a relative plateau.The invasion quantity increasing with the cells concentration increased.The increasing slowed down when the cell concentration reached 3.0×10^5/m1.Under the identical conditions,the invasion ability of the cells with the capacity of bone marrow metastasis than the cells without ability of bone marrow metastasis.Conclusions： The transwell chamber for the prostatic carcinoma cells were successfully established.It will be crucial for future study of the invasive ability of the prostatic carcinoma cells.