摘要
目的:探讨伏隔核微注射orexin-A后,大鼠摄食和活动的变化。方法:采用SD大鼠(250-280g),用脑立体定位仪在伏隔核植入微量注射管。大鼠随机分组,分别微注射乳酸格林液(Ringer's),orexin-A 100pmol和500pmol。观察微注射后大鼠0-1h,1-2h,2-4h摄食和0-30min,30-60min,60-90min,90-120min活动性变化。结果:Orexin-A微注射后,大鼠0-1h,1-2h摄食量增加;30-60min,60-90min,90-120min的活动性显著增加(P<0.05 vs对照组)。结论:伏隔核是orexin-A刺激大鼠增加摄食量,提高其活动性的作用点。
Objective： To investigate the effect of orexin-A on feeding and locomotor activity in the accumbens shell （Accsh）. Methods： SD rats （250-280） were used and implanted a guide cannulae into the accumbens shell （AccSh） by stereotaxic instrument. Then rats were randomly divided into three group and microinjected Ringer＇s solution, 100 pmol and 500 pmol orexin-A respectively. The feeding were recorded in 0-1h, l-2h,2-4h and locomotor activity were recorded in 0-30 min, 30-60min,60-90min,90-120min after micin- jection. Results： Orexin-A augmented feeding in the 0-1 h and 1-2 h and stimulated locomotor activity in the 30-60 min, 60-90 min, and 90-120 min post-infusion （P 〈 0.05 vs control group）. Conclusion： AccSh is a site of orexin A modulation of feeding behavior and loco- motor activity.
出处
《现代生物医学进展》
CAS
2012年第9期1621-1623,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金资助项目(30772075)
