摘要
目的:探讨仙人掌多糖对体外肺鳞癌细胞(SK-MES-1)生长抑制作用。方法:体外培养SK-MES-1细胞,四甲基偶氮唑盐(MTT)法观察野生仙人掌多糖对其生长的抑制,计算最低抑制浓度及抑制率;观察不同浓度多糖对细胞形态的影响;SDS-PAGE凝胶电泳简析不同组别蛋白质表达的差异。结果:野生仙人掌多糖24 h和48 h对肿瘤细胞的最低抑制浓度和抑制率分别为0.0625 mg/ml.34.06%和0.0625 mg/ml 35.37%;不同组间蛋白质表达有差异。结论:野生仙人掌多糖对SK-MES-1肺鳞癌细胞有抑制作用。
Objective： To investigate the anti-tumor effect of wild Cactus polysaccharide on SK-MES-1 lung squamous carcinoma cells. Methods： SK-MES-I cells were cultivated in vitro. MTT assay was used to detect the inhibition phenomena of wild Cactus polysac- charide on the growth of tumor cells, calculated the lowest inhibition concentration and tumor inhibitive ratio（IR）. The influence of differ- ent concentrations of polysaccharide on cell shape was examined respectively. SDS-PAGE assay was used to estimate the difference of protein expression among each group. Results： The lowest inhibition concentration and IR of wild Cactus polysaccharide to SK-MES-1 for 24 h and 48h were 0.0625 mg/ml, 34.06% and 0.0625 mg/ml, 35.37%. The protein expression was different in each group. Conclu- sion： Wild Cactus polysaccharide had anti-tumor effect on lung squamous carcinoma cells.
出处
《现代生物医学进展》
CAS
2012年第9期1651-1654,共4页
Progress in Modern Biomedicine
