T-bet、GATA-3与T细胞亚群在再障中的相关性研究

Expression of T-bet, GATA-3 mRNA and T cell subsetsin in peripheral blood of patients with aplastic anemia

目的:探讨外周血中T-bet和GATA-3 mRNA的表达在再生障碍性贫血中的发病机制及意义。方法:入选27例再障患者,其中重型再障15例,轻型再障l2例,25例健康体检者为对照组。采用流式细胞术检测参试者外周血Thl和Th2细胞,RT-PCR检测外周血单核细胞中转录因子T-bet和GATA-3 mRNA的表达。结果:与健康对照组相比,再障患者血浆T-bet mRNA与Th1细胞比例显著升高(P<0.01),GATA-3 mRNA与Th2细胞明显降低(P<0.05、P<0.01)。与轻型再障患者相比,重型再障患者血浆转录因子T-bet mRNA及Th1细胞比例均明显升高(P<0.01),GATA-3 mRNA水平与Th2细胞比例也显著下降(P<0.05、P<0.01)。结论:T-bet与GATA-3异常表达可增强Th1细胞功能,抑制Th2细胞功能,导致患者免疫功能异常,最终引起再障发生、发展。

Objective：To investigate the pathogenic role of T-bet and GATA-3 mRNA expression in aplastic anemia（AA）.Methods： 27 patients with AA including 15 cases of severe AA and 12 cases of mediate AA,and 25 healthy people（control group） were selected.Flow cytometry was used to detect the expression of Thl and Th2 in peripheral blood,and RT-PCR to quantify T-bet and GATA-3 mRNA.Results： Compared with the control group,the expression of Thl and T-bet mRNA in the AA group increased significantly（P〈0.01）,while the expression Th2 and GATA-3 mRNA decreased（P〈0.05,P〈0.01）.Conclusion： Abnormal expression of T-bet and GATA-3 plays an important role in the development and progression of AA,with the possible mechanism of enhancing Thl cells and inhibting Th2 cells.