荧光定量PCR测定血清前列腺特异性膜抗原在前列腺癌诊断中的意义

The meaning of mensurate prostatic specific membrane antigen （PSMA） in the serum by fluorescent quantitation PCR in the diagnosis of prostate cancer

目的用荧光定量PCR方法测定血清前列腺特异性膜抗原（PSMA）的表达强度。评价其在前列腺癌诊断中的敏感性和特异性。方法分别提取前列腺癌患者和良性前列腺增生患者的血清标本，通过RT—PCR方法扩增目标基因组，同时用荧光标记物与目标基因组中特定部位结合，循环特定次数后用荧光定量PCR仪测定荧光标记物的浓度，从而了解PSMA在两组患者中的表达。通过和前列腺特异性抗原（PSA）比较来了解PSMA在前列腺癌诊断中的价值。结果前列腺癌患者血清中的PSMA表达高于良性前列腺增生患者的表达。结论PSMA是较PSA敏感性和特异性更高的检测前列腺癌的指标，为临床早期筛选前列腺癌提供更强的依据。

Objective To observe the expression of prostatic specific membrane antigen （PSMA） in the serum in order to get the sensitivity and differential of PSMA in the diagnosis of prostate cancer. Methods Distill the serum speciments from the patients of Pea and BPH, enlarge the target genes with RT-PCR, at the same time combine the fluorescent marker with the specifically part of the target genes, after circlae the certain times, we can read the concentration of fluorescent marker with the machine, and observe the difference expression of PSMA in two terms, to compare the value of PSMA and PSA in the diagnosis of prostate cancer. Results The expression of PSMA in the serum of prostate cancer was higher than that in the serum of benign prostate hyperplasia. Conclusions PSMA is more sensitivity and differential than PSA in the diagnosis of prostate cancer, and provide stronger evidence for filtering prostate cancer in the early stage.