苯佐卡因和乙二醇苯醚对企鹅珍珠贝麻醉效果的研究

Anaesthesia effect of two anaesthetic chemicals,2-phenoxyethanol and benzocaine,on the winged pearl oyster(Pteria penguin(Rding))

为了寻找企鹅珍珠贝Pteria penguin(Rding)植核时安全与有效的麻醉方法,本实验比较了乙二醇苯醚和苯佐卡因对企鹅珍珠贝植核时的麻醉效果。结果表明,海水温度在25℃时,φ=0.1%,0.2%,0.3%的乙二醇苯醚与ρ=500,800,1 000 mg.L-1的苯佐卡因都有麻醉效果;随着麻醉剂用量的增加,企鹅珍珠贝麻醉所需时间减少,复苏时间变长;在一定剂量下,随水温的上升(21～27℃),麻醉和复苏时间均减少;φ=0.2%的乙二醇苯醚、ρ=800 mg.L-1和1 000 mg.L-1的苯佐卡因对企鹅珍珠贝均具有较好的麻醉效果,30min内的麻醉效果均达到100%,麻醉贝复苏快,且1周后均存活。

Anaesthetics, as a means of reducing stress reaction, may have a role in the pearl seeding process. Anaesthetic chemicals were identified to facilitate the safe and effective anesthetization of wing pearl oysters （ Pteria penguin （Rtiding） ） in seeding. Two anaesthetic chemicals, 2-phenoxyethanol and henzocaine, were assessed for their ability to relax the wing pearl oyster. These reagents of different concentrations were added into containers of the oysters to observe their reactions at the temperatures ranging from 21,23, 25 and 27 ℃. At 25 ℃ 2-phenoxyethanol of 0.1% -0. 3% （v/v） and benzocaine of 500, 800, 1 000 mg·L-1 appeared promising. And 2-phenoxyethanol and benzocaine were selected to determine their effects on the time to relaxation and to recovery and on survival of the oysters after 7 days of relaxation. It was found that as anesthetic concentrations of the reagents increased, the time to relaxation of the oysters decreased while the time to recovery tended to increase. Increased duration of exposure to the relaxants increased the time to recovery. When the temperature increased both the time to relaxation and to recovery of the oysters decreased. The highest proportion of the relaxed oysters （ 100% ） within 30 rain and the shortest exposure time required for anaesthesia were recorded in the treatments of the oysters with 0.2% 2-phenoxyethanol or 1 000 mg·L-1 benzocaine, and the treated oysters had no mortalities after 7 days of the treatment.