4种外源激素对长春花生物碱积累的影响

Effects of exogenous hormones on the accumulation of alkaloids in Catharanthus roseus(L.)

分别用茉莉酸、乙烯利、乙酰水杨酸、色氨酸等4种试剂的不同质量浓度的溶液,处理温室盆栽的长春花成苗和幼苗。在处理2 d后,测定长春花叶片中的吲哚生物总碱、长春碱、长春质碱和文多灵碱的含量。结果表明,4种试剂对长春花植物中吲哚生物碱的积累有明显的促进作用。

Four chemical reagents, jasmonic acid （JA） , ethrel, Aspirin and tryptophan, were used as exogenous hormones to treat pot plants of Catharanthus roseus grown in green house to determine the contents of medicinal components, alkaloids, in C. roseus leaves. The pot plants at the age of 2 and 5 months were treated with the reagents at different concentrations. Two days after treatment, the contents of catharanthine, vinblastine and vindoline in leaves were determined by using HPLC. All the reagents under trial obviously improved the accumulation of indole alkaloids including catharanthine and vinblastine in the leaves of C. roseus, and the optimum treatment concentrations of these reagents were determined. These reagents apparently promote the accumulation of total indole alkoloids in the leaves of C. roseus.