甲基强的松龙诱导大鼠胸腺淋巴细胞凋亡的研究

Study of methylprednisolone inducing rat thymocyte apoptosis

目的 探讨大剂量甲基强的松龙 (MP)诱导大鼠胸腺淋巴细胞凋亡作用的存在。方法 采用流式细胞仪技术、琼脂糖凝胶电泳等方法对细胞凋亡状态进行检测。结果 大剂量MP注于大鼠体内后 ,可诱导大鼠胸腺淋巴细胞进入凋亡状态 ,注入后 6h ,无明显细胞凋亡 ,12h后出现凋亡改变 ,2 4h凋亡细胞改变明显增加 ,连续用药 2～ 3d ,虽凋亡细胞比率增加 ,但与用药 2 4h相比 ,差异无显著性。结论 甲基强的松龙体内注射后 ,以用药后 2 4h诱导胸腺淋巴细胞凋亡的作用为最强。

Objective To detect the rat thymocyte apoptosis after treating the rats with MP.Methods The DNA cleavage percent was measured by flow cytometry and sepharose electrophoresis.Results ① Large dose injection of MP could induce the apoptosis of rat thymocyte.The DNA cleavage percent increased significantly ( P <0 01).② At hour 12 after injection of large dose of MP the apoptosis increased,and at hour 24 the apoptosis was highest.Conclusion MP can induce thymus dependent lymphocyte apoptosis.