小鼠体外受精胚胎发育的研究

Study on the development of controlled ovarian hyperstimulation mouse embryo in vitro fertilization

目的:通过对不同培养方式下促排小鼠体外受精卵发育能力的比较研究和分析,为新建生殖医学实验室培养环境质量控制提供依据。方法:在相同的培养条件和培养时间下,将获得的小鼠卵母细胞随机分为两组,体外受精后,分别以四孔培养法(群体培养)和微滴培养法(单独培养)进行培养,观察记录并计算双原核卵率、24h≥4细胞率、48h融合胚胎率以及96h囊胚率。结果:四孔培养法体外培养后24h≥4细胞数胚胎率明显高于微滴培养法,有统计学意义(P<0.01);48h融合胚发育率有统计学意义(P<0.05);囊胚形成率上差异无统计学意义。结论:结果显示,四孔培养法(群体培养)更利于体外受精小鼠胚胎的发育。

Objective： To provide a basis of environmental quality control for the new reproductive medicine laboratory by comparing and analysising the controlled ovarian hyperstimulation mouse zygotes viability in different culture methods.Methods： The mouse oocytes cultured in the same condition at the same time were randomly divided into two groups.After in vitro fertilization,one group was cultured in 4-well dish（culturing in groups）,and the other group in micro-drops（culturing individually）.The rate of two-pronuclear eggs,≥ 4 cell on 24h rate,morula rate on 48h and blastocyst embryos on 96h were observed and calculated.Results： There was significant difference（P0.01） on the cleavage rate of ≥ 4-cell on 24h between 4-well culture and micro-drop systems,besides,the morula rate on 48h was also significantly different（P0.05）.However,the blastocyst rate had no statistically significant difference.Conclusion： The results indicated that 4-well dish（culturing in groups） is more conducive to the development of mouse embryos in vitro fertilization.