高效液相色谱法测定全血环孢霉素A的浓度

Determination of Cyclosporine Whole Blood by High Performance Liquid Chromatography

建立了高效液相色谱法测定全血环孢霉素Ａ 浓度的方法。全血样品经多步处理后以２０ μＬ注入ｒｅｏｓｏｌｖｅ Ｃ１８，５μ色谱柱，采用Ｖ（乙腈）∶Ｖ（水）＝ ８０∶２０ 的流动相，在７５℃柱温下，以１ ｍ Ｌ／ｍ ｉｎ 流速洗脱，检测波长为２０８ｎｍ 。环孢霉素Ａ 的回收率为９９％ ～１０４ ％ ，相对标准偏差＜ ３．４２％ （ｎ＝ ５） ，日间＜ ８．１９％ （ｎ＝ ４） 。最低检测浓度为２５ μｇ／Ｌ。

A HPLC method for the assay of cyclosporine A in whole blood was estabtished.After multistep pretreatment ,20 μL sample was injected into reversed phase column (200 mm ×4.6 mm,5 μm,C 18 ) at 75℃,eluted with a mobile phase of acetonitrile/water(80∶20) at a flow rate of 1.0 mL/min and monitored at 208 nm.The analytical recovery was 99 %～104% and inter day and intra days RSD was below 3.4% and 8.2% respectively.The detection limit was 25 μg/L.The method provided excellent recovery and good precision,and was accurate.No interference was observed.