血管内皮生长因子在鼻内翻性乳头状瘤中的表达

The significance and expression of vascular endothelial growth factor on nasal inverted papillomas

目的 :明确血管内皮生长因子 ( VEGF)及其受体在鼻内翻性乳头状瘤 ( NIP)组织中的表达 ,探讨其在 NIP发病中的作用。方法 :采用免疫组化 SP法 ,对 1 1例 NIP标本及 6例慢性单纯性鼻炎患者的下鼻甲标本 ,检测 VEGF及其胎肝激酶受体 ( flk- 1 ) ,以图像分析仪半定量测定 VEGF和 flk- 1的表达强度。结果 :VEGF在 NIP的上皮细胞和血管内皮细胞的表达 ,均显著高于下鼻甲组织相应部位 (均 P <0 .0 1 )。flk- 1在 NIP血管内皮细胞的表达显著高于下鼻甲组织相应部位( P <0 .0 0 1 )。 flk- 1表达于 NIP上皮细胞而未见于下鼻甲上皮。结论 :VEGF参与了 NIP的病理过程 ,表现为部分 NIP组织富含血管 ,手术时易出血。VEGF的较高水平表达 ,可能在间质水肿的发生过程中亦发挥了重要作用。 NIP上皮细胞 VEGF的表达水平有可能作为衡量 NIP恶变的指标之一。

Objective:To determine the expression of vascular endothelial growth factor (VEGF) and its receptor in nasal inverted papillomas (NIP) and to clarify the function of VEGF in the establishment of NIPs.Method:VEGF and its receptor,fetal liver kinase 1 (flk 1) expression were exam ined by immunohistochemistry using SP method in sections of NIPs from 11 patients and inferior turbinates from 6 patients with chronic simple rhinitis.An automatic image analyzer was used to detect the staining results.Gray scale,a half quantitative parameter,was used to describe the expression of VEGF and flk 1.Result:It whowed that all the epithelium in NIPs,together with vascular and stroma adjacent to the epithelium,expressed different degree of VEGF.The VEGF expression in epithelium was significantly stronger in NIPs than that in inferior turbinates (P< 0.001 ).The expressions of both VEGF and flk 1 in vascular endothelium were more intense in NIPs than that in inferior turbinates (P< 0.001 ,P< 0.001 ).Flk 1 positive expression was observed in epithilum of NIPs but not in inferior turbinates.Conclusion:The results suggest that VEGF participate in the growth of NIPs,part of which shows the characteristics of containing plenty of blood vessels and being bloody in operation.The enhanced expression of VEGF may also play a key role in the development of edematous stroma.The expression degree of VEGF in the epithelium may be identified as one of the parameters of judging the propensity of NIPs′ malignant transformation.