新疆汉族和维吾尔族鼻咽癌组织的PCR和免疫组化检测比较

Detection of EBV DNA,EBNA2 and LMP-1 in Tumor Tissues of Han and Uygur Patients with Nosopharyngeal Carcinoma in Xinjiang

目的 :了解新疆汉族维吾尔 (维 )族鼻咽癌 (NPC)发病因素的差别。 方法 :用 PCR与免疫组化技术对73例 NPC组织 (汉族 4 1例、维族 3 2例 )分别进行 EB病毒脱氧核糖核酸 (EBV-DNA )、EB病毒核抗原(EBNA2 )、潜伏膜蛋白 (LMP- 1 )检测。结果 :EBV- DNA阳性检出率汉族为 48.8% (2 0 /41 ) ,维族59.4% (1 9/32 ) ;EBNA2 表达汉族 43.9% (1 8/41 ) ,维族 43.8% (1 4 /32 ) ,两民族间无显著性差异(P >0 .0 5)。 LMP- 1表达 ,汉族 34.1 % (1 4 /41 ) ,明显低于维族 71 .9% (2 3/32 ) (P <0 .0 5)。在EBV- DNA阳性病例中 ,LMP- 1表达汉族为 40 .0 % (8/2 0 ) ,也明显低于维族 78.9% (1 5/1 9) (P<0 .0 5) ;EBNA2 表达两民族间无统计学差异 (P >0 .0 5)。结论 :新疆 NPC的发生与 EB病毒感染有相关性 ;LMP- 1在维族鼻咽上皮细胞恶性转化过程中 ,具有较汉族更重要的作用。

Objective:To investigate the possible difforences of human nosopharyngeal carcinogenesis between Han and Uygur patients in xinjiang.Method:Detection of EBV DNA,EBNA2 and LMP 1 in tumor tissues of 73 patients (Han 41,uygur 32) with nosopharyngeal carcinoma (NPC) were peroformed by PCR and immunohistochemistry.Result:Tho positive rate of EBV DNA,EBNA2 expression was 48.2 %(20/41), 43.9 %(18/41) in Han and 59.3 %(19/32), 43.7 %(14/32) in Uygur respectively,no significant difference was found (P> 0.05 ).Expression of LMP 1 in NPC,in both positive and negative EBV DNA specimens,was higher in Uygur ( 78.9 %) than that in Han (40%) (P< 0.05 ),Conclusion:The EBV infection may involve in nosophayyngoal carcinogonosis in xinjiang;the LMP 1 of EBV could be a more critical factor in malignant transformation of normal nosopharyngeal epithelium in uygur people than in han Chinese.