摘要
目的探讨基于核糖体基因26SrDNA D1/D2区序列分析法在临床酵母菌菌种鉴定中的应用。方法收集来源于复发性外阴阴道念珠菌病分泌物标本93株,PCR扩增其26SrDNA D1/D2区,对扩增产物进行序列测定和分析,并与基因库中的基因序列进行同源性比对。结果所有菌株均鉴定到种,同源性达99%和100%,同属于真菌双核亚界、子囊菌门、酵母菌科的3个属,89株为candida,3株为Kodamaea,1株为Pichia。其中candida中有7个种,38株candida glabrata,23株candida albicans,16株candida parapsolisis,9株candida metapdilosis,1株candida orthop-silisis,1株candida tropicalis,1株candida nivariensis;3株Kodamaea ohmeri;1株Pichia kudriavzevii。结论复发性外阴阴道念珠菌病的病原体主要为candida属的candida glabrata、candida albicans和candida parapsolisis,非candidaalbicans占75.27%是其特征;26SrDNA D1/D2区序列分析为临床酵母菌的分子水平鉴定提供了一种准确、可行的方法。
Objective To explore the ribosomes gene based on 26S rDNA D1/D2 area sequence analysis method in the clinical application of yeast strain identification.Methods To collect from recurrent genital vaginal moniliosis secretion specimens of 93 plants,PCR amplification its 26S rDNA D1/D2 area,the amplification products series were determined and analyzed,and the genetic sequences with gene pool than homology.Results All strains are identification to the kind,the homology of 99% and 100%,belong to the fung,dikarya,ascomycota,saccharomycetaceae of this three genera,89 strains for candida,3 strains for Kodamaea,for Pichia 1 strain.One of seven kinds of candida,38 strains candida glabrata,candida albicans 23 strains,16 strains candida parapsolisis,9 of candida metapdilosis,candida orthopsilisis 1 strain,candida tropicalis 1 strain,1 strain candida nivariensis;3 strains Kodamaea ohmeri;Pichia kudriavzevii 1 strain.Conclusion Recurrent genital vaginal moniliosis pathogen of candida mainly for candida glabrata,candida albicans and candida parapsolisis,the candida albicans are take 75.27% of its characteristic;26S rDNA D1/D2 area series analysis for clinical yeast molecular level appraisal provides a accurate and feasible method.
出处
《中国实验诊断学》
2012年第4期625-628,共4页
Chinese Journal of Laboratory Diagnosis
基金
广东省人口与计生委科研立项项目(2009308)
深圳市龙岗区科技计划医疗卫生立项项目(YL-2009030)
