免疫组织化学法和流式细胞术检测非小细胞肺癌骨髓微转移

Detection of micrometastases in rib marrow of patients with non-small lung cancer using immunohistochemistry and flow cytometry

目的 流式细胞和免疫组织化学技术检测非小细胞肺癌骨髓微转移状况的差异比较.方法 选取2004年3月至2007年5月我院手术治疗的非小细胞肺癌160例,术前未经化疗及放疗,术中抽取肋骨骨髓,分别应用流式细胞技术和免疫组化法检测骨髓中阳性细胞表达率.结果 160例非小细胞肺癌患者免疫组化检测骨髓转移阳性率为30.0％（48例）,流式细胞术检测骨髓转移阳性率为34.4％（55例）.112例免疫组化检查阴性的患者中,流式细胞术检测发现12例患者骨髓中存在微转移；而48例免疫组化检查阳性的患者中,有5例流式细胞术检测为阴性.结论 流式细胞术和免疫组化技术都可检测非小细胞肺癌骨髓微转移,流式细胞技术有着更高的敏感性和检测效率.

Objective To detect the bone marrow micrometastasis （BMM）of patients with non-small lung cancer（NSCLC） and to compare the result between using flow cytometry and immunohistochemistry. Methods From April 2004 to May 2007, 160 cases of NSCLC who were not treated with chemotherapy and radiotherapy in our hospital but undergoing surgical treatment for NSCLC were prospectively investigated for the presence of BMMs by u- sing immunohistochemistry and flow cytometry. Borie marrow aspiration was done during the operation. Results Fifty-five of 160 patients（34.4% ） with NSCLC were found to have BMM using flow cytometry while 30.0% were found using immunohistochemistry. The 12 samples were positive for the flow cytometric assay but for the immunocy- tochemical test there were 112 negative samples. The 48 samples were positive for the immunocytochemical test but for the flow cytometric assay there were 5 negative samples. Conclusions Flow cytometric detection of BMM corre- lates with the immunohistochemical detection, and its level of sensitivity is greater than that of immunohistochemical staining. Compared with immunocytochemistry, flow cytometry is quick and cost effective.