全反式维甲酸对高糖培养的人肾小球足细胞synaptopodin蛋白表达的影响

Effect of all trans-retinoic acid on the expression of synaptopodin in human podocytes induced by high glucose

目的 探讨高糖培养的人肾小球足细胞synaptopodin蛋白表达的变化以及全反式维甲酸对其表达的干预效应.方法 以体外培养的条件性永生人肾小球足细胞为研究对象,平均分为正常糖（NG）组、高糖（HG）组、HG＋5tmol/L全反式维甲酸干预组、HG＋ 15 μmol/L全反式维甲酸干预组.蛋白质印迹法检测HG组足细胞培养0、3、6、9d时和其他各组足细胞培养9 dsynaptopodin蛋白的表达.结果 HG组足细胞培养3、6、9d时synaptopodin蛋白表达灰度值与培养0d时比较明显降低[（1.15±0.04）％、（0.78±0.04）％、（0.41±0.05）％比（1.49±0.07）％,P＜0.05].培养9d后,与NG组比较,HG组、HG＋5μmol/L维甲酸干预组、HG＋ 15 μmol/L维甲酸干预组足细胞synaptopodin蛋白表达灰度值均明显下降[（0.41±0.05）％、（0.86±0.04）％、（0.90±0.07）％比（1.47 ±0.08）％,均P＜0.05].结论 高糖可以使体外培养的足细胞synaptopodin蛋白表达下降,全反式维甲酸具有潜在的保护足细胞和防治糖尿病肾病的作用.

Objective To investigate the effect of all trans-retinoic acid on the expression of synaptopodin in human podocytes induced by high glucose. Methods Conditionally immortalized human podocytes were exposed to nomal glucose group （NG） and high glucose group （HG）. The expression of synaptopodin in podocytes was detected by Western blot. Results The expression of synaptopodin decreased significantly in time-dependent manner after 3, 6 and 9 days of high glucose （HG） stimulation [ （ 1.15 ± 0. 04） %, （0. 78 ± 0. 04） %, （0. 41 ± 0. 05 ） % vs （ 1.49 ± 0.07 ） % , P 〈 0.05 ]. Compared to HG group, 5 μmol/L and 15μmol/L all-trans retinoic acid （ATRA） increased synaptopodin expressionat 9th day dramaticaly [ （0.41 ± 0.05 ） %, （0.86 ± 0.04） %, （0.90 ± 0.07） % vs （ 1.47 ± 0.08） %, P 〈 0.05 ]. Conclusions High concentration of glucose decreases the expression of synaptopodin in podocyte. All trans retinoic acid（ATRA）may exert protective role on diabetic nephropathy by increasing synaptopodin expression which is downregulated by HG.