脑蛋白水解物中多肽组分的高效毛细管电泳分析

High performance capillary electrophoresis analysis of polypeptide in protein hydrolysis ingredients of brain tissue

目的 利用高效毛细管电泳法比较2种水解方法提纯的脑蛋白水解物中的多肽组分,同时监测进口脑蛋白水解物注射液的多肽组分分布.方法 采用固相萃取法提取脑组织中的多肽成分,以PACE/A5500型高效毛细管电泳仪对进口脑蛋白水解物注射液和本实验室提取的脑组织匀浆物进行比较.结果 进口脑蛋白水解物注射液中多肽成分大部分集中在＞14400区域,本实验室提纯的脑蛋白水解物成分中多肽多集中在相对分子质量6000 ～ 14 400区域,且纯度及含量均较高.结论 高效毛细管电泳法可有效地对脑蛋白水解物注射液中多肽组分进行分析和比较,2种提纯方法对脑蛋白的裂解能力相差不大.

Objective To extract the protein hydrolysis ingredients of brain tissue were extracted and to analyze the components of polypeptide in cerebrolysin by using the method of high-performance capillary electrophoresis （HPCE）. Methods Brain homogenate were extracted by solid phase extraction method. Cerebrolysin and brain tissue hydrolyzated extracted in lab were detected by HPCE （ PACE/AS500, Beckman）. Result The result of HPCE showed that the cerebrolysin injections molecular weight of most polypeptides were more than 14 400. The pu- rity and content of cerebrolysin was better and the molecular weights were mainly between 6000 and 14 400. Conclusion The method of HPCE can be effectively used in analyzing components of polypeptides of brain tissue hydrolyzated. There is no difference of protein hydrolysis ingredients of brain tissue extracted by 2 kinds of extraction methods.