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冷冻保存对富血小板纤维蛋白组织形态与生物活性因子的影响 被引量：4

Effects of crypreservation on platelet-rich fibrin
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摘要 目的 观察超低温冷冻保存方法对富血小板纤维蛋白（PRF）组织形态与生物活性因子的影响.方法 取健康志愿者的肘静脉血提取PRF标本,液氮保存1周,分别进行光镜、透射电镜观察和免疫组化分析,并与新鲜PRF对比.结果 光镜下冷冻前后的PRF无明显差异,免疫组化检测冷冻前后血小板源性生长因子（PDGF）、转化生长因子β1 （TGF-β1）、白细胞介素-1（IL-1）、IL-4、肿瘤坏死因子（TNF）均有阳性反应表达,其中PDGF、TGF-β1、IL-1、TNF表达差异无统计学意义（P＞0.05）,冻后PRF中IL-4表达高于新鲜PRF,差异有统计学意义[平均光密度值（0.207±0.006）比（0.125±0.015）,P＜0.05].透射电镜显示,冻后PRF中大部分血小板外形不规则或有膜损伤.结论 冷冻保存对PRF生物活性因子和纤维蛋白结构无明显影响,仍可保持其促进组织愈合和抗炎反应的作用. Objective To investigate the effects of cryopreservation on the morphology and bioactive factors of platelet-rich fibrin（PRF）. Methods PRF was carried out at - 196 ~C for one week and investigated by light microscope. Transmission electron microscope （TEM） and the bioactive factors in PRF were analyzed before and af- ter the cryopreservation. Results PRF had no significant changes in light microscope before and after cryopreserva- tion. The immuno-histochemical results showed that Platelet derived growth factor （PDGF）, transforming growth factor beta 1 （ TGF beta 1 ）, interleukin 1 （ IL-1, IL-4 ） and tumor necrosis factor （TNF） were expressed both in fresh and frozen PRF. The express of PDGF ,TGF-β1, IL-1, TNF in frozen PRF was not significantly increased compared with fresh PRF（ P 〉 0.05 ） , while the express of IL-4 in frozen PRF increased significantly（ P 〈0. 05 ）. Conclusion The fibrin net-structure and bioactive factors of frozen PRF are not significantly destroyed by cryopreservation treatment.
作者 李丹 张剑明 罗晓丁 韩哲
机构地区 天津医科大学口腔医院种植科
出处 《中国医药》 2012年第5期629-631,共3页 China Medicine
关键词 富血小板纤维蛋白 冷冻保存 超微结构 生长因子 Platelet-rich fibrin Cryopreservation Uhrastructure Growth factor
分类号 R78 [医药卫生—口腔医学]
  • 相关文献

参考文献11

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二级参考文献10

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共引文献65

同被引文献60

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引证文献4

二级引证文献29

中国医药
2012年 第5期

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