天女木兰嫩茎愈伤组织诱导及再生体系建立研究

Study on callus induction and establishment of regeneration system for Magnolia sieboldii

为了保护濒危植物天女木兰,采用植物组织培养方法,以嫩茎为材料,进行愈伤组织诱导与分化、不定芽生根与试管苗生根继代增殖的培养,以及试管苗移栽与定植的研究,建立起天女木兰再生体系技术。结果表明:MS+ZT0.4mg/L+2,4-D2.5mg/L是嫩茎愈伤组织诱导培养和继代增殖培养的理想培养基;MS+GA30.5mg/L+BA0.6mg/L是愈伤组织分化培养的理想培养基;把不定芽用10mg/L的IAA溶液处理24h后,接种到1/3MS+IBA0.6mg/L+0.8DA-60.5mg/L+NAA0.5mg/L培养基上的生根培养方法是不定芽生根培养和试管苗生根继代增殖培养的理想方法;在温室中试管苗易移栽成活,定植的试管苗保持了野生天女木兰的所有植物学性状。

The study aimed to preserve the endangered plant of Magnolia sieboldii. By using tissue culture methods, the tender stems of Magnolia sieboldii were used as materials to do the research on callus induction and differentiation, adventitious buds rooting and rooting of the subculture, tube seedlings transplanting colonization, and establishment of regeneration system technique of Magnolia sieboldii. The results show that MS ＋ ZT 0.4 mg/L＋ 2,4-D 2.5 mg/L is the ideal medium for callus induction and subculture; MS ＋ GA3 0.5 mg/L ＋ BA 0.6 mg/L is the best medium for callus differentiation. After dealt with IAA （concentration of 10 rag/L） for 24 hours, the adventitious buds could be easily induced rooting in the medium of 1/3 MS ＋ IBA 0.6 mg/L＋0.8 DA-6 0.5 rag/L＋ NAA 0.5 mg/L. In the greenhouse, the tube seedlings could be easy to survive after transplanting. The colonization of plantlets maintained all biological traits which wild plant has.