植物凝集素亲和色谱层析法分离缺糖基转铁蛋白诊断酒精性肝病的临床研究

Clinical study of separating carbohydrate deficient transferrin (CDT) using by plant lectin and affinity chromatography on diagnosis of alcoholic liver disease

目的:观察酒精性肝病患者血清缺糖基转铁蛋白(CDT)水平变化,探讨CDT诊断酒精性肝病的临床价值。方法:应用植物凝集素亲和层析法分离血清CDT和正常转铁蛋白(Tf),然后应用ELISA技术分别测定酒精性肝病组患者及正常对照组人员CDT和Tf,最后计算CDT/Tf值。结果:两组人员血清植物凝集素亲和层析洗脱图谱均显示两个洗脱峰;CDT存在于第一洗脱峰,Tf存在于第二洗脱峰,ELISA定量结果显示:酒精性肝病组患者血清CDT平均水平为5.05±1.64,Tf平均水平为54.91±40.94,CDT/Tf值为0.123,正常对照组人员血清CDT平均水平为4.78±1.03,Tf平均水平451.61±241.37,CDT/Tf值为0.0117,经统计学分析,P<0.05,差异有显著性意义。结论:酒精性肝病患者血清CDT水平及CDT/Tf值明显升高,CDT是诊断酒精性肝病良好的生化标志。

Objective： To observe patients with alcoholic liver carbohydrate deficient transferrin （CDT） level and investigate the diagnosis value of carbohydrate deficient transferrin in alcoholic liver disease. Methods： Carbohydrate deficient transferfin （CDT） and normal transferrin （Tf） in serum were isolated by lectin affinity chromatography, and then CDT and Tf in alcoholic liver disease and control groups were determined by ELISA, finally CDT / Tf value was calculated. Results： Serum lectin affinity chromatography elution profiles in alcoholic liver disease group and control group showed two elution 15eaks, CDT was found in the first elution peak, Tf consisted in the second elution peak, ELISA quantitative results showed the average value of CDT and Tf were respectively 5.05 ± 1.64 and 54. 91 ± 40. 94, CDT / Tf value wasl2. 30% in the alcoholic liver disease group; in the control group the average value of CDT and Tf were respectively 4. 78 ± 1.03 and 451.61 ± 241.37, CDT / Tf was 1.17%. Conclusion ： Patients with alcoholic liver carbohydrate deficient transferrin （CDT） level and the value of CDT / Tf increased obviously. CDT is a valuable biochemical marker in diagnosis of alcoholic liver disease .