芦荟大黄素对血吸虫病性肝纤维化小鼠的影响

Effect of aloeemodin on liver fibrosis induced by Schistosoma Japonicum infections in mice

目的:探讨芦荟大黄素对血吸虫肝纤维化的影响。方法:采用日本血吸虫尾蚴感染小鼠建立血吸虫性肝纤维化模型,用芦荟大黄素0.3mg.kg-1.d-1治疗8周。测定小鼠肝脏匀浆丙二醛(MDA)水平,免疫组化检测其肝组织转化生长因子β1(TGF-β1)、血管内皮生长因子(VEGF)、局部黏着斑激酶(FAK)、Ⅰ型胶原和Ⅲ型胶原的表达。结果:芦荟大黄素能减轻血吸虫性肝纤维化的组织病理改变,使血吸虫肝纤维化小鼠肝脏Ⅰ型胶原和Ⅲ型胶原的表达降低,显著抑制肝组织中TGF-β1、VEGF和FAK的表达(P<0.01)。结论:芦荟大黄素对血吸虫性肝纤维化有治疗作用,其作用机制可能与影响TGF-β1、VEGF和FAK的表达相关。

Objective： To observe the effect of aloeemodin on liver fibrosis mice in duced by Schistosoma Japonicum. Methods： Mice＇liver fibrosis model was induced by Schistosoma Japonicum infection for 8 weeks. Suspension of aloeemodin prepared with normal saline was given orally to the mice, O. 3 mg per mouse every day for 8 weeks. The level of malon- dialdehyde （MDA） in liver homogenate was detected. The level of transforming growth factor beta 1 （TGF-β1） , vascular endothelial growth factor （ VEGF）, Focal adhesion kinase （FAK） and type I , III collagen in liver tissue were detected by immuno- histochemistry. Results： The pathological changes of liver tissue was inpreved by aloeemodin. The level of type I and III collagen in schistosomiasis liver fibrosis mice were decreased. TGF-β1, VEGF and FAK expression levels were increased in liver fibrosis induced by Schistosoma Japonicum. However, TGF-β1, VEGF and FAK expression were inhibited after administration of aloeemodin. Conclusion： Aloeemodin might have therapeutic effects on liver fibrosis induced by Schistosoma of liver through the effects of TGF-β1, VEGF and FAK expression.